Hi ive been getting the same problems with twonky 4.3 running on readynas NV+, it just comes up all the time with "The selected content is not supported by this Media Player" So what i did tried every possible combination I had and can only see that it could be the way twonky is dealing with the files.

My hardware renderer is a sonos zp80 (tested with the sonos desktop controller and works with that from my nas share, Not Upnp it searches network shares directly on the sonos system)

Then i tried
nevo server->nevosl->Sonos (all good so far)
nevo server->Nevosl->nevo player (Still all good)
PS3 accessing twonky share on my nas (totally fine)
Xbox 360 and that was fine

here's where the problems start =

Twonkymedia 4.3 on readynas->nevosl->sonos (not supported file types)
Twonkymedia 4.3 on readynas->nevosl->Nevo Player (Not supported file types)

so then I went to see if there was a beta on the twonkymedia forum for my readynas and the link for my machine seems to be the only one not working for Twonkymedia 4.4 Beta 5 (Or any other beta for that mater). So then I thought I would download the the beta for windows and put it on my macbook pro to test. could find the server in nevo studio, uploaded to nevo sl and the zone never becomes active(Doh), so i think ok its a beta try the 4.3 on my windows machine still doesnt become active.

Ive now deleated the twonky from my macbook. But now I am officially at a dead end untill someone can show me the light. Sorry for the long post but i started on this at 1pm today (when my sonos zoneplayer was delivered) and its now 1:35am. hope someone can give me some inspiration to continue before all this mass of shiny boxes and a pretty remote end up out the window. Thanks lee.